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Handbook of Microbiological Media, Fourth Edition part 182 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Transgrow Medium 1805 Use For the cultivation and maintenance of Pseudomonas species. TPT 18 Medium DSMZ Medium 1127 Composition per liter Glucose. 0.5g Yeast extract.0.1g Pancreatic digest of casein.0.1g MgSO4-7H2O.0.05g CaCl2-2H2O.0.02g pH 6.0 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Adjust pH to 6.0. Use For the cultivation of Mucilaginibacter gracilis. TPY Medium Composition per liter Pancreatic digest of casein.10.0g Glucose.5.0g Pancreatic digest of soybean meal.5.0g Yeast extract.2.5 g K2HPO4.2.0g Agar.1.5g Cysteine-HCl.0.5g MgCl26H2O.0.5g ZnSO4-7H2O.0.25g CaCl2.0.15g FeCl3.1.0pg Tween 80.1.0mL pH 6.5 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the isolation and cultivation of Bifidobacterium species. TPYG Medium Composition per liter Pancreatic digest of casein.10.0g Peptone.5.0g Yeast extract.5.0g Glucose solution.50.0mL Glucose Solution Composition per 100.0mL Glucose.20.0g Preparation of Glucose Solution Add glucose to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except glucose solution to distilled deionized water and bring volume to 950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add 50.0mL of sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation of Clostridium felsineum. Trace Elements Solution HO-LE Composition per liter H3BO3.2.85g MnCl24H2O.1.8g Sodium tartrate.1.77g FeSO4-7H2O. 1.36g CoCl26H2O.0.04g CuCl2.2H2O.0.027g Na2MoO4-2H2O.0.025g ZnCl2. 0.02g Preparation of Trace Elements Solution HO-LE Add components to distilled deionized water and bring volume to
