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Handbook of Microbiological Media, Fourth Edition part 177 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thiobacillus caldus Medium 1755 Thiobacillus caldus Broth Composition per liter Solution A.960.0mL Solution D.20.0mL Solution B.10.0mL Solution C.10.0mL pH 2.5 0.2 at 25 C Solution A Composition per 960.0mL Na2SO4-10H2O.3.2g NH4 2SO4.3.0g MgSO4-7H2O.0.5g KCl.0.1g K2HPO4.50.0mg Preparation of Solution A Add components to distilled deionized water and bring volume to 960.0mL. Mix thoroughly. Adjust pH to 1.75 with H2SO4. Autoclave for 15 min at 15 psi pressure-121 C. Solution B Composition per 10.0mL FeCl3-6H2O.11.0mg Ca NO3 2-4H2O.10.0mg H3BO3.2.0mg MnSO4 H2O.2.0mg ZnSO4-7H2O.0.9mg Na2MoO4-2H2O.0.8mg CoCl2-6H2O.0.6mg CuSO4-5H2O.0.5mg Preparation of Solution B Add components to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Solution C Composition per 10.0mL Glucose.0.45g Preparation of Solution C Add glucose to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Solution D Composition per 20.0mL Na2S4O6.0.77g Preparation of Solution D Add Na2S4O6 to distilled deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Aseptically combine 960.0mL of sterile solution A with 10.0mL of sterile solution B 10.0mL of sterile solution C and 20.0mL of sterile solution D. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the heterotrophic cultivation and maintenance of Thiobacillus caldus. Thiobacillus caldus Broth Composition per liter Solution A.970.0mL Solution C.20.0mL Solution B.10.0mL pH 2.5 0.2 at 25 C Solution A Composition per 970.0mL Na2SO4-10H2O.3.2g NH4 2SO4.3.0g MgSO4-7H2O.0.5g KCl.0.1g K2HPO4.50.0mg Preparation of Solution A Add components to distilled deionized water and bring volume to 970.0mL. Mix thoroughly. Adjust pH to 1.75 with H2SO4. Autoclave for 15 min at 15 psi pressure-121 C. Solution B Composition per 10.0mL FeCl3-6H20.11.0mg Ca NO3 2-4H2O.10.0mg H3BO3.2.0mg MnSO4-H2O.2.0mg ZnSO4 7H2O.0.9mg Na2MoO4-2H2O.0.8mg
