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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Vascular endothelial growth factor-A and chemokine ligand (CCL2) genes are upregulated in peripheral blood mononuclear cells in Indian amyotrophic lateral sclerosis patients | Gupta et al. Journal of Neuroinflammation 2011 8 114 http www.jneuroinflammation.eom content 8 1 114 JJOURNAL1 OF. NEUROINFLAMMATION SHORT REPORT Open Access Vascular endothelial growth factor-A and chemokine ligand CCL2 genes are upregulated in peripheral blood mononuclear cells in Indian amyotrophic lateral sclerosis patients Pawan K Gupta1 Sudesh Prabhakarf Chandrika Abburi Neel K Sharma and Akshay Anand Abstract Background We have earlier shown that protein levels of vascular endothelial growth factor-A VEGF-A and chemokine ligand-2 CCL2 were elevated in Indian amyotrophic lateral sclerosis ALS patients. Here we report the mRNA levels of VEGF-A and CCL2 in Indian ALS patients since they display extended survival after disease onset. Methods VEGF-A and CCL2 mRNA levels were measured in peripheral blood mononuclear cells PBMCs of 50 sporadic Indian ALS patients using Real Time Polymerase Chain Reaction PCR and compared with normal controls n 50 . Their levels were adjusted for possible confounders like cigarette smoking alcohol and meat consumption. Results VEGF-A and CCL2 mRNA levels were found to be significantly elevated in PBMCs in ALS patients as compared to controls. PBMCs from definite ALS revealed higher VEGF-A mRNA expression as compared to probable and possible ALS. CCL2 mRNA levels were found to be unaltered when definite probable and possible ALS were compared. PBMCs from patients with respiratory dysfunction showed much higher VEGF-A and CCL2 elevation when compared to patients without respiratory dysfunction. No association of smoking alcohol and meat consumption with VEGF-A and CCL2 was observed after analyzing the data with univariate and multivariate analysis. Conclusion VEGF-A and CCL2 mRNA upregulation in PBMCs may have a clinico-pathological etiological epidemiological association with ALS pathogenesis. The cross-cultural and cross-ethnic investigations of these molecules could determine if they have any role in enhancing the mean survival .
