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Handbook of Microbiological Media, Fourth Edition part 170 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | TB Nitrate Reduction Broth 1685 Preparation of Bovine Albumin Solution Add bovine albumin to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.8 with NaOH. Store at -20 C. Preparation of Medium Add components except bovine albumin solution and glucose solution to distilled deionized water and bring volume to 900.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Add 50.0mL sterile glucose solution and 50.0mL bovine albumin solution. Mix thoroughly. Aseptically distribute into sterile tubes. Use For the cultivation of Mycobacterium tuberculosis. TB HiVeg Broth Base with Bovine Serum and Glucose Composition per liter Plant peptone No. 3.4.0g Na2HPO4.2.5g Yeast extract.2.0g Sodium citrate.1.5g KH2PO4.1.0g MgSO4.0.6g Polysorbate 80.0.5g Glucose solution.50.0mL Bovine serum.50.0mL pH 7.0 0.2 at 25 C Source This medium without glucose or bovine albumin is available as a premixed powder from HiMedia. Glucose Solution Composition per 100.0mL Glucose.10.0g Preparation of Glucose Solution Add glucose to distilled deion-ized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except bovine serum and glucose solution to distilled deionized water and bring volume to 900.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Add 50.0mL sterile glucose solution and 50.0mL bovine serum. Mix thoroughly. Aseptically distribute into sterile tubes. Use For the cultivation of Mycobacterium tuberculosis. TB HiVeg Broth Base without Tween 80 with Bovine Albumin and Glucose Composition per liter Plant peptone No. 3. 4.0g Na2HPO4.2.5g Yeast extract.2.0g Sodium citrate.1.5g KH2PO4.1.0g MgSO4.0.6g Glucose solution.50.0mL Bovine albumin solution.50.0mL pH 7.0 0.2 at 25 C Source This medium without glucose or bovine albumin is available as a premixed powder from HiMedia. Glucose Solution .
