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Handbook of Microbiological Media, Fourth Edition part 168 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Sulfurospirillum MV Medium 1665 Preparation of Trace Elements Solution SL-10 Add FeCl2-4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 80 N2 20 CO2. Vitamin Solution Composition per liter Pyridoxine-HCl.10.0mg Thiamine-HCl-2H2O.5.0mg Riboflavin.5.0mg Nicotinic acid.5.0mg D-Ca-pantothenate.5.0mg p-Aminobenzoic acid.5.0mg Lipoic acid.5.0mg Biotin.2.0mg Folic acid.2.0mg Vitamin B12.0.1mg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80 H2 20 CO2. Filter sterilize. Preparation of Medium Prepare and dispense medium under 80 N2 20 o CO2. Add components except NaHCO3 solution Na2S-9H2O solution cysteine solution NaNO3 solution Na-lactate solution and vitamin solution to distilled deionized water and bring volume to 1.0mL. Mix thoroughly. Sparge with 80 N2 20 CO2. Adjust pH to 7.3. Dispense either 10.0mL aliquots into 15mL Hungate tubes or 50.0mL aliquots into 100mL Hungate bottles. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically inject from sterile stock solutions NaHCO3 solution Na2S-9H2O solution cysteine solution vitamin solution sodium nitrate solution and sodium lactate solution. Final pH should be 7.3. Use For the cultivation of Sulfurospirillum arsenophilum Geospirillum spy and Sulfurospirillum barnesii. Sulfurospirillum MV Medium DSMZ Medium 1097 Composition per liter NaCl .20.0g MgCl26H20.3.0g CaCl22H2O.0.8g KCl.0.7g NH4Cl .0.2g KH2PO4.0.2g Resazurin .0.5mg Vitamin solution.10.0mL Sulfide solution.10.0mL Fumarate solution.10.0mL Bicarbonate solution.10.0mL Trace elements solution SL-10.2.0mL Selenite-tungstate solution.1.0mL pH 7.2 0.2 at 25 C Bicarbonate Solution Composition per 10.0mL NaHCO3.2.5g Preparation of Bicarbonate Solution Add NaHCO3 to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 20 CO2 80 N2. .
