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Handbook of Microbiological Media, Fourth Edition part 155 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Salmonella Differential Agar 1535 Salinibacter ruber Medium DSMZ Medium 936 Composition per liter NaCl.195.0g MgSO4-7H2O.49.5g MgCl2-6H20. 34.6g KCl.5.0g CaCl22H2O.1.25g Yeast extract.1.0g NaBr.0.625g NaHCO3.0.25g pH 7.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Salinibacter ruber. Salinivibrio costicola Subspecies vallismortis Medium DSMZ Medium 597 Composition per liter NaCl.25.0g MgSO4-7H2O.9.6g MgCl26H2O.7.0g Glucose.5.0g KCl.3.8g Yeast extract.1.0g CaCl22H2O.0.5g K2HPO4-3H2O.0.4g NaHCO3 solution.100.0mL pH 7.0 0.2 at 25 C NaHCO3 Solution Composition per 100.0mL NaHCO3.3.0g Preparation of NaHCO3 Solution Add NaHCO3 to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except NaHCO3 solution to distilled deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Aseptically add 100.0mL NaHCO3 solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation of Salinivibrio costicola subsp. vallismortis. Salmonella Chromogen Agar Rambach Equivalent Agar Composition per liter Agar.15.0g Peptone.5.0g NaCl.5.0g Yeast extract.2.0g Meat extract.1.0g Sodium deoxycholate.1.0g pH 7.3 0.2 at 25 C Source This medium is available from Fluka Sigma-Aldrich. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes. Use A differential diagnostic agar for the detection of Salmonella in food including the isolation and enumeration of Salmonella from bivalves. Salmonella Chromogenic Agar OSCM Composition per liter Chromogenic mix . 28.0g Agar.12.0g .
