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Handbook of Microbiological Media, Fourth Edition part 150

Handbook of Microbiological Media, Fourth Edition part 150 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Regan-Lowe Semisolid Transport Medium 1485 Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into screwcapped tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the transport and isolation of bacteria from dental plaque especially Streptococcus mutans Streptococcus sanguis and Lactobacillus species. Reduced Transport Fluid Composition per liter NH4 2SO4.9.0g NaCl.9.0g K2HPO4.4.5g KH2PO4.4.5g Na2CO3.4.0g EDTA ethylenediamine tetraacetic acid .3.8g Dithiothreitol.2.0g MgSO4-7H2O.1.8g pH 8.0 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Aseptically distribute into sterile tubes with rubber stoppers. Use For the transport and isolation of bacteria from dental plaque especially Streptococcus mutans and Streptococcus sanguis. Also used for the cultivation of a variety of Gram-positive bacteria from the oral cavity especially streptococci actinomycetes lactobacilli clostridia Bacteroides species Fusobacterium species and Veillonella species. Reduced Transport Fluid Composition per liter Stock mineral salt solution No. 1.75.0mL Stock mineral salt solution No. 2.75.0mL Dithiothreitol 1 solution .20.0mL Ethylenediamine tetraacetic acid 1M solution .10.0mL Na2CO3 8 solution .5.0mL Resazurin 0.1 solution .1.0mL pH 8.0 0.2 at 25 C Stock Mineral Salt Solution No. 1 Composition per 100.0mL K2HPO4.0.6g Preparation of Stock Mineral Salt Solution No. 1 Add K2HPO4 to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Stock Mineral Salt Solution No. 2 Composition per 100.0mL NaCl.1.2g NH4 2SO4.1.2g K2HPO4.0.6g MgSO4-7H2O.0.25g Preparation of Stock Mineral Salt Solution No. 2 Add components to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.