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Handbook of Microbiological Media, Fourth Edition part 149 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | R70-2 Broth Modified with Glucose 1475 Nicotinic acid.5.0mg Calcium pantothenate.5.0mg p-Aminobenzoic acid.5.0mg Thioctic acid.5.0mg Biotin.2.0mg Folic acid.2.0mg Cyanocobalamin.0.1mg Preparation of Wolfe s Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. 100X Modified Salts Composition per liter CaCl2-2H2O.1.47g FeCl3-6H2O.0.27g ZnSO4-7H2O.0.144g MnSO4-H2O.0.085g CoCl26H2O.0.024g NiCl26H2O.0.024g Na2MoO4-2H2O.0.024g CuSO4-5H2O.0.016g HCl concentrated.4.1mL Preparation of 100X Modified Salts Add components to dis-tilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except Wolfe s vitamin solution and 100X modified salts to distilled deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 5.0. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile Wolfe s vitamin solution and 100X modified salts. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation of Acetobacter xylinum. R70-2 Broth Modified with Fructose Composition per liter Fructose.30.0g Yeast extract.5.0g Dimethyl glutaric acid.4.01g NH4 2SO4.3.3g Trisodium citrate-2H2O.1.18g KH2PO4.1.0g MgSO4-7H2O.0.25g Wolfe s vitamin solution.10.0mL 100X modified salts.10.0mL pH 5.0 0.2 at 25 C Wolfe s Vitamin Solution Composition per liter Pyridoxine-HCl.0.01g Thiamine-HCl.5.0mg Riboflavin.5.0mg Nicotinic acid.5.0mg Calcium pantothenate.5.0mg p-Aminobenzoic acid.5.0mg Thioctic acid.5.0mg Biotin.2.0mg Folic acid.2.0mg Cyanocobalamin.0.1mg Preparation of Wolfe s Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. 100X Modified Salts Composition per liter CaCl2-2H2O.1.47g FeCl3-6H2O.0.27g ZnSO4-7H2O.0.144g MnSO4-H2O.0.085g CoCl26H2O.0.024g NiCl26H2O.0.024g Na2MoO4-2H2O.0.024g .
