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Handbook of Microbiological Media, Fourth Edition part 141 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Phthalic Acid Medium 1395 Yeast extract.2.5 g CaCO3.1.0g NH4Cl.0.3g Mg2SOq.0.3g FeCl2.0.01g pH 7.0 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Shewanella hanedai Alteromonas hane-dai. Photobacterium Broth Composition per liter NaCl.30.0g Sodium glycerol phosphate.23.5g Pancreatic digest of casein.5.0g KH2PO4.3.0g Yeast extract.2.5g CaCO3.1.0g NH4Cl.0.3g MgSO4-7H2O.0.3g FeCl3.0.01g pH 7.0 0.2 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks to form a shallow layer of medium. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and demonstration of luminescence by photobacteria. For the cultivation and maintenance of Alteromonas hanedai Photobacterium phosphoreum Shewanella hanedai Vibrio fischeri Vibrio harveyi and other Vibrio species. Photobacterium Broth Composition per liter Seawater aquarium salt.33.0g Tris hydroxymethyl aminomethane.6.0g NH4Cl.5.0g Pancreatic digest of casein.5.0g Yeast extract.5.0g Glycerol.3.0g pH 7.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Acetobacter pasteurianus. Photobacterium HiVeg Broth Composition per liter NaCl.30.0g Sodium glycerophosphate.23.5g Plant hydrolysate.5.0g KH2PO4.3.0g Yeast extract.2.5g CaCO3.1.0g NH4Cl.0.3g MgSO4.0.3g FeCl3.0.01g pH 7.0 0.2 at 25 C Source This medium is available as a premixed powder from HiMedia. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. .