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Handbook of Microbiological Media, Fourth Edition part 133 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Nutrient Gelatin 1315 Nutrient Broth Sodium Chloride Thymine Medium Composition per liter Pancreatic digest of gelatin.5.0g NaCl.5.0g Beef extract.3.0g Thymine.0.03g pH 7.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Salmonella choleraesuis. Nutrient Broth Salts Medium Composition per liter Pancreatic digest of gelatin.5.0g Beef extract.3.0g NaCl.3.0g MgSO4-7H2O.0.2g CaCl2-2H2O.0.15g MnSO4-H2O.0.05g pH 6.8 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Bacillus subtilis. Nutrient Broth Standard II Composition per liter Special peptone.8.6g NaCl.6.4g pH 7.5 0.1 at 37 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of a variety of fastidious and nonfastidious microorganisms. Nutrient Broth 1 2 Strength Composition per liter Pancreatic digest of gelatin.2.5g Beef extract.1.5g pH 6.9 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Cytophaga allerginae Cytophaga allergi-nae and Thermomonospora chromogena. Nutrient Broth with Streptomycin Composition per liter Peptone.5.0g Beef extract.3.0g Streptomycin solution.10.0mL pH 6.8 0.2 at 25 C Streptomycin Solution Composition per 10.0mL Streptomycin.0.02g Preparation of Streptomycin Solution Add streptomycin to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. .
