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Handbook of Microbiological Media, Fourth Edition part 129 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Natroniella Medium 1275 Trace Elements Solution SL-6 Composition per liter MnCl2-4H2O.0.5g H3BO3.0.3g CoCl2-6H2O.0.2g ZnSO4-7H2O. 0.1g Na2MoO4-2H2O. 0.03g NiCl26H2O.0.02g CuCl2-2H2O.0.01g Preparation of Trace Elements Solution SL-6 Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium Add components except solution A solution B and trace elements solution SL-4 to distilled deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add 100.0mL of sterile solution A 3.0mL of sterile solution B and 1.0mL of sterile trace elements solution SL-4. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation of Pseudomonas putida. Natranaerobius Medium DSMZ Medium 1095 Composition per liter NaCl.100.0g Yeast extract.10.0g Tryptone.10.0g NH4Cl .0.5g KH2PO4.0.2g MgCl26H2O.0.1g Sucrose solution.10.0mL Vitamin solution.10.0mL Cysteine solution.10.0mL Bicarbonate solution.10.0mL Carbonate solution.10.0mL Trace elements solution SL-10.1.0mL pH 8.5 0.2 at 25 C Vitamin Solution Composition per liter Pyridoxine-HCl.10.0mg Thiamine-HCl-2H2O.5.0mg Riboflavin.5.0mg Nicotinic acid.5.0mg D-Ca-pantothenate.5.0mg p-Aminobenzoic acid.5.0mg Lipoic acid.5.0mg Biotin.2.0mg Folic acid. 2.0mg Vitamin B12 . 0.1mg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80 H2 20 CO2. Filter sterilize. Trace Elements Solution SL-10 Composition per liter MgSO4-7H2O.3.0g Nitrilotriacetic acid.1.5g NaCl.1.0g MnSO4-2H2O.0.5g CoSO4-7H2O.0.18g ZnSO4-7H2O.0.18g CaCl2-2H2O.0.1g FeSO4-7H2O.0.1g NiCl26H2O.0.025g KAl SO4 212H2O.0.02g H3BO3.0.01g Na2MoO4-4H2O.0.01g CuSO4-5H2O.0.01g Na2SeO3-5H2O.0.3mg Preparation of Trace Elements Solution SL-10 Add nitrilotriacetic acid to 500.0mL of distilled deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled .
