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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Comparison of metal-dependent catalysis by HIV-1 and ASV integrase proteins using a new and rapid, moderate throughput assay for joining activity in solution | AIDS Research and Therapy BioMed Central Open Access Methodology Comparison of metal-dependent catalysis by HIV-1 and ASV integrase proteins using a new and rapid moderate throughput assay for joining activity in solution Mark D Andrake11 Joseph Ramcharant2 George Merkel1 Xue Zhi Zhao3 Terrence R Burke Jr3 and Anna Marie Skalka 1 Address Institute for Cancer Research Fox Chase Cancer Center 333 Cottman Avenue Philadelphia PA 19111 USA 2Locus Pharmaceuticals Inc Blue Bell PA USA and 3Laboratory of Medicinal Chemistry Center for Cancer Research National Cancer Institute Frederick MD 21702 USA Email Mark D Andrake - mark.andrake@fccc.edu Joseph Ramcharan - jramcharan@locuspharma.com George Merkel - george.merkel@fccc.edu Xue Zhi Zhao - zhaox@NCI.FCRF.gov Terrence R Burke - tburke@helix.nih.gov Anna Marie Skalka - AM_Skalka@fccc.edu Corresponding author fEqual contributors Published 29 June 2009 Received 10 April 2009 AIDS Research and Therapy 2009 6 14 doi 10.1186 1742-6405-6-14 Accepted 29 June 2009 This article is available from http www.aidsrestherapy.cOm content 6 1 14 2009 Andrake et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background HIV-1 integrase IN is an attractive target for the development of drugs to treat AIDS and inhibitors of this viral enzyme are already in the clinic. Nevertheless there is a continuing need to devise new approaches to block the activity of this viral protein because of the emergence of resistant strains. To facilitate the biochemical analysis of wild-type IN and its derivatives and to measure the potency of prospective inhibitory compounds a rapid moderate throughput solution assay was developed for INcatalyzed joining of viral and target DNAs based on the detection of a .
