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Handbook of Microbiological Media, Fourth Edition part 118 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Middlebrook 7H12 Medium 1165 patients with secondary antitubercular drugs. Generally these strains fail to grow on 7H10 medium. Middlebrook 7H11 HiVeg Agar Base with Middlebrook ADC Enrichment Composition per liter Agar.15.0g Na2HPO4.1.5g KH2PO4.1.5g Plant hydrolysate.1.0g L-Glutamic acid.0.5g NH4 2SO4.0.5g Sodium citrate.0.4g MgSO4.0.05g Ferric ammonium citrate.0.04g Pyridoxine.1.0mg Malachite green.1.0mg Biotin.0.5mg Middlebrook ADC enrichment.100.0mL Glycerol.5.0mL pH 6.6 0.2 at 25 C Source This medium without glycerol and Middlebrook ADC enrichment is available as a premixed powder from HiMedia. Middlebrook ADC Enrichment Composition per 100.0mL Bovine albumin fraction V.5.0g Glucose.2.0g Catalase.0.003g Preparation of Middlebrook ADC Enrichment Add components to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add glycerol to 900.0mL of distilled deionized water and add remaining components except Middlebrook ADC enrichment. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Aseptically add 100.0mL of sterile Middlebrook ADC enrichment. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation of drug-resistant isoniazid INH strains of Mycobacterium tuberculosis. For the cultivation of particularly fastidious strains of tubercle bacilli that occur following treatment of tuberculosis patients with secondary antitubercular drugs. Middlebrook 7H11 HiVeg Agar Base with Middlebrook OADC Enrichment Composition per liter Agar.15.0g Na2HPO4.1.5g KH2PO4.1.5g Plant hydrolysate.1.0g L-Glutamic acid. 0.5g NH4 2SO4.0.5g Sodium citrate . 0.4g MgSO4.0.05g Ferric ammonium citrate. 0.04g Pyridoxine.1.0mg Malachite green.1.0mg Biotin.0.5mg Middlebrook OADC enrichment.100.0mL Glycerol.5.0mL pH 6.6 0.2 at 25 C Source This medium without glycerol and Middlebrook OADC enrichment is available as a premixed powder
