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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Role of miR-1 and miR-133a in myocardial ischemic postconditioning | He et al. Journal of Biomedical Science 2011 18 22 http www.jbiomedsci.eom content 18 1 22 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Role of miR-1 and miR-133a in myocardial ischemic postconditioning D I L . I_I 1 Ỷ I I I 2 Ỷ A I J L-. -J 0 S-. 2 . . r -J -7 L . L . -J 2 I_I 7 I . -. 4 ft I p. r L . 5 o I y I 6 v I L . -J s 7 Din He Jian Xiao An-Jing Ren Yu-Feng Zhang Hao Zhang Min Chen Ding Ale Xiao-Gang Gao Ying-Wei Wang1 Abstract Background Ischemic postconditioning IPost has aroused much attention since 2003 when it was firstly reported. The role of microRNAs miRNAs or miRs in IPost has rarely been reported. The present study was undertaken to investigate whether miRNAs were involved in the protective effect of IPost against myocardial ischemia-reperfusion IR injury and the probable mechanisms involved. Methods Thirty SD rats weighing 250-300 g were equally randomized to three groups Control group where the rats were treated with thoracotomy only IR group where the rats were treated with ischemia for 60 min and reperfusion for 180 min and IPost group where the rats were treated with 3 cycles of transient IR just before reperfusion. The extent of myocardial infarction LDH and CK activities were measured immediately after treatment. Myocardial apoptosis was detected by TUNEL assay. The myocardial tissue was collected after IR or IPost stimulation to evaluate the miRNAs expression level by miRNA-microarray and quantitative real-time RT-PCR. Realtime PCR was conducted to identify changes in mRNA expression of apoptosis-related genes such as Bcl-2 Bax and Caspase-9 CASP9 and Western blot was used to compare the protein expression level of CASP9 in the three groups. The miRNA mimics and anti-miRNA oligonucleotides AMO were transferred into the cultured neonatal cardiomyocytes and myocardium before they were treated with IR. The effect of miRNAs on .
