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Handbook of Microbiological Media, Fourth Edition part 116 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Methylophaga alcalica Agar 1145 Preparation of Carbonate Solution Add Na2CO3 to distilled de-ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Thiosulfate Solution Composition per 10.0mL NaS2O3-5H2O.18.0g Preparation of Thiosulfate Solution Add NaS2O3-5H2O to dis-tilled deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Acetate Solution Composition per 10.0mL Sodium acetate.0.2g Preparation of Acetate Solution Add sodium acetate to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Preparation of Medium Add components except acetate vitamin carbonate thiosulfate and magnesium sulfate solutions to distilled de-ionized water and bring volume to 960.0mL. Mix thoroughly. Distribute into closed vessels with a medium to headspace ratio of 1 5 to 1 10. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Aseptically add the acetate vitamin carbonate thiosulfate and magnesium sulfate solutions. Use For the cultivation with faster growth rates of Methylonatrum spp. Methylophaga Agar Composition per 103.0mL Agar solution.50.0mL Mineral base 2X.50.0mL Solution T.2.0mL Vitamin B12 solution.1.0mL Methanol.0.3mL pH 7.3 0.2 at 25 C Agar Solution Composition per 500.0mL Agar.15.0g Preparation of Agar Solution Add agar to distilled deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Mineral Base 2X Composition per 500.0mL NaCl.24.0g MgCl26H2O.3.0g MgSO4-7H2O.2.0g CaCl22H2O.1.0g KCl.0.5g Bis-Tris buffer bis 2-hydroxyethyl amino-tris hydroxymethyl -methane .0.5g Wolfe s mineral solution.10.0mL Preparation of Mineral Base 2X Add components to distilled deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 7.3. Autoclave for 15
