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Handbook of Microbiological Media, Fourth Edition part 115 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Methanospirillum SK Medium 1135 CuSO4-5H2O.0.01g H3BO3.0.01g Preparation of Trace Minerals Solution Add nitrilotriacetic acid to 500.0mL of distilled deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Mix thoroughly. Add distilled deionized water to 1.0L. Adjust pH to 7.0. Vitamin Solution Composition per liter Vitamin B12.0.1g Pyridoxine-HCl.10.0mg p-Aminobenzoic acid.5.0mg Lipoic acid.5.0mg Nicotinic acid.5.0mg Riboflavin.5.0mg Thiamine-HCl.5.0mg Biotin.2.0mg Folic acid.2.0mg Vitamin B12.0.4mg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. L-Cysteine Na2S Solution Composition per liter L-Cysteine-HCl.1.25g Na2S-7H2O.1.25g Preparation of L-Cysteine Na2S Solution Gently heat and bring 75.0mL of distilled deionized water to boiling. Add L-cysteine-HCl. Adjust pH to 10.0 with 3N NaOH. Add Na2S-9H2O. Mix thoroughly. Gently heat and bring to boiling. Cool to room temperature while sparging with 100 N2. Bring volume to 100.0mL with distilled deionized water. Gently heat and bring to boiling. Cool to 60 C while sparging with 100 N2. Anaerobically distribute into serum bottles. Autoclave for 15 min at 15 psi pressure-121 C. FeSO4-6H2O Solution Composition per 100.0mL FeSO4-6H2O.0.2g Preparation of FeSO4-6H2O Solution Add FeSO4-6H2O to dis-tilled deionized water and bring volume to 100.0mL. Mix thoroughly. Resazurin Solution Composition per 10.0mL Resazurin.10.0mg Preparation of Resazurin Solution Add resazurin to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Preparation of Medium Prepare and dispense medium under 80 H2 20 CO2. Add components except Na2CO3 Na2CO3 solution and L-cysteine Na2S solution to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Continue boiling for 3 min. Cool to 60 C while sparging with 80 H2 20 CO2. Add Na2CO3 and L-cysteine Na2S solution. Mix thoroughly. .
