Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Handbook of Microbiological Media, Fourth Edition part 111 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Methanococcus sp. Medium 1095 Sodium Acetate Solution Composition per 100.0mL Sodium acetate-3H2O.13.6g Preparation of Sodium Acetate Solution Add 13.6g of sodium acetate-3H2O to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Pantoyllactone Solution Composition per 100.0mL Pantoyllactone.0.013g Preparation of Pantoyllactone Solution Add pantoyllactone to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Iron Stock Solution Composition per 100.0mL Fe NH4 2 SO4 2-6H2O.0.2g Preparation of Iron Stock Solution Add Fe NH4 2 SO4 2-6H2O to 5.0mL of distilled H2O containing 2 drops of concentrated HCl. Mix thoroughly. When the Fe NH4 2 SO4 2-6H2O has dissolved bring the volume to 100.0mL with distilled deionized water. Resazurin Solution Composition per 10.0mL Resazurin.10.0mg Preparation of Resazurin Solution Add resazurin to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Preparation of Medium Prepare and dispense medium under 80 H2 20 CO2. Add components except NaHCO3 and Na2S-9H2O solution to distilled deionized water and bring volume to 1080.0mL. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 3 min. Cool to room temperature while sparging with 80 H2 20 CO2. Add NaHCO3. Mix thoroughly. Anaerobically distribute 9.8mL volumes into anaerobic tubes. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically add 0.2mL of sterile Na2S-9H2O solution to each tube. Mix thoroughly. Use For the cultivation of Methanococcus species. Methanococcus Medium Composition per liter NaCl.18.0g Mg2SO4-7H2O.3.45g MgCl2-2H2O.2.75g Pancreatic digest of casein.2.0g Yeast extract.2.0g Sodium acetate.1.0g L-Cysteine-HCl.0.5g Na2S-9H2O.0.5g NH4HCO3.0.5g KCl.0.335g NH4Cl.0.225g CaCl22H2O.0.14g KH2PO4.0.14g Calcium DL-pantothenate.5.0mg Na2SeO3.2.0mg FeSO4-2H2O.1.0mg Resazurin.1.0mg Trace minerals stock solution.10mL pH 6.5 0.2 at 25 C Trace Minerals Stock Solution Composition per liter .
