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Handbook of Microbiological Media, Fourth Edition part 110 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Methanobacterium II Medium 1085 use inject for each 5.0mL medium 0.2mL sterile NaHCO3 solution 0.05mL sterile Na2S-9H2O solution and 0.05mL sterile methanol solution. The pH should be 7.0. Use For the cultivation of Methanosarcina barkeri DSM 10131 and Methanosarcina mazei Methanococcus mazei Methanosarcina frisia DSM 10132. Methanobacterium II Medium DSMZ Medium 825 Composition per 1065.0mL Yeast extract.1.0g NH4Cl.1.0g NaCl.0.6g Cysteine-HCl-H2O.0.5g Sodium acetate.0.5g K2HPO4.0.3g KH2PO4.0.3g MgCl26H2O.0.2g CaCl22H2O.0.1g KCl.0.1g Resazurin.0.5mg NaHCO3 solution.40.0mL Na-formate solution.15.0mL Trace elements solution.10.0mL Vitamin solution.10.0mL Na2S-9H2O solution.10.0mL pH 7.0 0.2 at 25 C Na-Formate Solution Composition per 10.0mL Na-formate . 5.0g Preparation of Na-Formate Solution Add Na-formate to dis-tilled deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Trace Elements Solution Composition per liter MgSO4-7H2O.3.0g Nitrilotriacetic acid.1.5g NaCl.1.0g MnSO4-2H2O.0.5g CoSO4-7H2O.0.18g ZnSO4-7H2O.0.18g CaCl22H2O.0.1g FeSO4-7H2O.0.1g NiCl26H2O.0.025g KAl SO4 212H2O.0.02g H3BO3.0.01g Na2MoO4-4H2O.0.01g CuSO4-5H2O.0.01g Na2SeO3-5H2O.0.3mg Preparation of Trace Elements Solution Add nitrilotriacetic acid to 500.0mL of distilled deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled deionized water to 1.0L. Mix thoroughly. Vitamin Solution Composition per liter Pyridoxine-HCl.10.0mg Thiamine-HCl-2H2O.5.0mg Riboflavin.5.0mg Nicotinic acid.5.0mg D-Ca-pantothenate.5.0mg p-Aminobenzoic acid.5.0mg Lipoic acid.5.0mg Biotin.2.0mg Folic acid.2.0mg Vitamin B12.0.1mg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80 H2 20 CO2. Filter sterilize. Na2S-9H2O Solution Composition per 10.0mL Na2S-9H2O.0.3g Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled .
