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Handbook of Microbiological Media, Fourth Edition part 108

Handbook of Microbiological Media, Fourth Edition part 108 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Membrane Clostridium perfringens Medium 1065 Lilly and Barnett Solution Composition per 100.0mL Fe NO3 3-9H2O.723.5mg ZnSO4-7H2O.439.8mg Preparation of Lilly and Barnett Solution Add components to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Preparation of Oligo Solution Combine 1.0mL of Lilly and Barnett solution and 0.66mL of 1 Hoagland solution. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of Agaricus xanthoderma Agaricus mac-rosporus Antrodia serialis Armillaria mellea Auricularia fuscosuc-cinea Boletinellus merulioides Boletus leucophaeus Cephaliophora irregularis Circinella umbellata Kuehneromyces mutabilis Laccaria laccata Lentinus tigrinus Lenzites betulina Leucogyrophana mollusca Lycoperdon foetidum Macrolepiota rhacodes Macrolepiota procera Pholiota lenta Phoma exigua and many other fungi. Melissococcus pluton Medium Composition per liter Glucose.10.0g Neopeptone.5.0g Peptone.2.5g Yeast extract.2.5g Soluble starch.2.0g Pancreatic digest of casein.2.0g L-Cysteine-HCl-H2O.0.25g Phosphate buffer 1M pH 6.7 .50.0mL pH 7.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Gently heat and bring to boiling. Distribute into tubes or flasks that have been flushed with 90 N2 10 CO2. Cap with butyl rubber stoppers. Place tubes in a press. Autoclave for 15 min at 15 psi pres-sure-121 C. Use For the cultivation and maintenance of Melissococcus pluton. Melissococcus pluton Medium Composition per liter Agar.20.0g KH2PO4.13.5g Glucose.10.0g Peptone. 10.0g Soluble starch.10.0g pH 6.6 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat .