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Handbook of Microbiological Media, Fourth Edition part 98 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Low Iron YC Agar 965 Vitamin K Solution Composition per 100.0mL Vitamin K1.1.0g Ethanol.99.0mL Preparation of Vitamin K Solution Add vitamin K1 to 99.0mL of absolute ethanol. Mix thoroughly. Preparation of Medium Add hemin and L-cystine to 5.0mL of NaOH. Mix thoroughly. Add remaining components except agar and gelatin. Bring volume to 750.0mL with distilled deionized water. Mix thoroughly. Gently heat and bring to boiling. In a separate flask add gelatin to 100.0mL of cold distilled deionized water. Gently heat and bring to 70 C. Add gelatin solution to the 750.0mL of basal medium. Mix thoroughly. Add agar. Bring volume to 1.0L with distilled deionized water. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes. Use For the cultivation of a wide variety of anaerobic bacteria. For the differentiation of anaerobic bacteria based on gelatinase production. After incubation of plates gelatinase activity is determined by the addition of Frazier s reagent. Bacteria that hydrolyze gelatin appear as colonies surrounded by a clear zone. Lombard-Dowell Neomycin Agar Egg Yolk Agar with Neomycin Composition per 9100.0mL Na2HPO4-12H2O.5.0g Glucose.2.0g Neomycin sulfate.0.1g LD Agar.9000.0mL Egg yolk emulsion.100.0mL MgSO4-7H20 5 solution .0.2mL pH 7.5 0.2 at 25 C LD Agar Composition per liter Agar.20.0g Pancreatic digest of casein.5.0g Yeast extract.5.0g NaCl.2.5g L-Cystine.0.4g L-Tryptophan.0.2g Na2SO3.0.1g Hemin. 10.0mg NaOH 1V NaOH .5.0mL Vitamin K1 solution.1.0mL Preparation of LD Agar Add hemin and L-cystine to 5.0mL of NaOH. Mix thoroughly. Add remaining components. Mix thoroughly. Gently heat and bring to boiling. Vitamin K Solution Composition per 100.0mL Vitamin K1.1.0g Ethanol.99.0mL Preparation of Vitamin K Solution Add vitamin K to 99.0mL of absolute ethanol. Mix thoroughly. Egg Yolk Emulsion Composition Chicken egg yolks.11 Whole chicken egg.1 Preparation of Egg Yolk Emulsion Soak eggs with 1 100 dilution of saturated mercuric chloride .
