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Handbook of Microbiological Media, Fourth Edition part 97 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Listeria Identification HiVeg Agar Base with PALCAMSelective Supplement 955 Listeria Enrichment HiVeg Broth Modified Composition per liter NaCl.20.0g Plant hydrolysate No. 1.10.0g Na2HPO4.9.6g Yeast extract.5.0g Plant extract.5.0g KH2PO4.1.35g Esculin.1.0g Nalidixic acid.0.02g Acriflavin hydrochloride Trypaflavin .0.012g pH 7.4 0.2 at 25 C Source This medium is available as a premixed powder from HiMe-dia. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the selective enrichment of Listeria species. Listeria Enrichment HiVeg Medium Base with Acriflavine and Nalidixic Acid UVM Composition per liter NaCl.20.0g Na2HPO4.12.0g Plant extract . 5.0g Plant hydrolysate. 5.0g Plant peptone No. 3. 5.0g Yeast extract. 5.0g KH2PO4.1.35g Esculin . 1.0g Nalidixic acid solution.1.0mL Acriflavine solution.1.0mL pH 7.4 0.2 at 25 C Source This medium without nalidixic acid or acriflavine solutions is available as a premixed powder from HiMedia. Nalidixic Acid Solution Composition per 10.0mL Nalidixic acid.0.2g NaOH 0.1M solution .10.0mL Preparation of Nalidixic Acid Solution Add nalidixic acid to 10.0mL of NaOH solution. Mix thoroughly. Filter sterilize. Acriflavine Solution Composition per 10.0mL Acriflavine.0.12g Preparation of Acriflavine Solution Add acriflavine to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Use freshly prepared solution. Preparation of Medium Add components except nalidixic acid solution and acriflavine solution to distilled deionized water and bring volume to 998.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add 1.0mL of sterile nalidixic acid solution. Mix thoroughly. Store at 4 C. Immediately prior to use aseptically add 1.0mL of sterile acriflavine .
