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Handbook of Microbiological Media, Fourth Edition part 44 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Clostridium thermosuccinogenes Medium 425 121 C. Aseptically add 50.0mL of sterile reductant solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation of Clostridium celerecrescens Clostridium papyrosolvens Clostridium stercorarium and Clostridium thermocel-lum. Clostridium thermohydrosulfuricum Medium Composition per liter Sucrose.10.0g Pancreatic digest of casein.10.0g Yeast extract.2.0g FeSO4-7H2O.0.2g Na2SO3.0.2g Na2S2O3-5H2O. 0.08g Resazurin.1.0mg pH 6.8 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100 N2 for 15 min. Autoclave for 30 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Clostridium thermohy-drosulfuricum Clostridium thermosaccharolyticum Thermoanaer-obacter ethanolicus and Thermoanaerobacter thermohydrosulfuricus. Clostridium thermolacticum Medium Composition per 1020.0mL KHCO3.4.5g NaCl.2.25g Sucrose. 2.0g Yeast extract. 2.0g MgSO4-7H2O.0.5g NH4Cl.0.5g K2HPO4.0.348g CaCl22H2O.0.25g KH2PO4.0.227g FeSO4-7H2O.2.0mg Resazurin.1.0mg L-Cysteine-HCl-H2O solution.10.0mL Na2S-9H2O solution.10.0mL Wolfe s vitamin solution.10.0mL Trace elements solution SL-6.3.0mL pH 7.0-7.2 at 25 C Wolfe s Vitamin Solution Composition per liter Pyridoxine-HCl.10.0mg p-Aminobenzoic acid.5.0mg Calcium pantothenate.5.0mg Nicotinic acid.5.0mg Riboflavin.5.0mg Thiamine-HCl.5.0mg Thioctic acid.5.0mg Biotin.2.0mg Folic acid. 2.0mg Cyanocobalamin.100.0pg Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Trace Elements Solution SL-6 Composition per liter H3BO3.0.3g CoCl2-6H2O.0.2g ZnSO4-7H2O.0.1g MnCl24H2O.0.03g Na2MoO4-H2O.0.03g NiCl26H2O.0.02g CuCl22H2O.0.01g Preparation of Trace Elements Solution SL-6 Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.4. L-Cysteine-HCTH2O Solution Composition per 10.0mL .
