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Handbook of Microbiological Media, Fourth Edition part 36

Handbook of Microbiological Media, Fourth Edition part 36 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Charcoal Agar 345 Solution 1 Composition per liter MgCl2-6H2O.40.0g KCl.4.0g CaCl22H2O.0.4g pH 7.4 0.2 at 25 C Preparation of Solution 1 Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Haloarcula vallismortis. Chaetomium Medium Composition per liter Agar.15.0g NaNO3.2.0g K2HPO4.1.2g MgSO4-7H2O.0.5g KCl.0.5g KH2PO4.0.14g Yeast extract.0.02g Fe2 SO4 3-H2O.0.01g pH 7.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Allow agar to cool. Place a strip of sterile filter paper on top of agar and inoculate filter strip. Use For the growth and maintenance of Chaetomium species. Chalquist s Antigen Medium Modified Composition per liter Soluble starch.0.5g Pancreatic digest of casein.0.05g L-Cysteine-HCl-H2O.0.01g NAD nicotinamide adeninedinucleotide .0.01g PPLO broth without Crystal Violet.90.0mL Swine serum inactivated.10.0mL Phenol Red 1 solution .0.25mL pH 7.6 0.2 at 25 C PPLO Broth without Crystal Violet Composition per 500.0mL Beef heart infusion from.11.52g Peptone.2.32g NaCl.1.15g Source PPLO broth without Crystal Violet is available as a premixed powder from BD Diagnostic Systems. Preparation of PPLO Broth without Crystal Violet Add components to distilled deionized water and bring volume to 500.0mL. Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Mycoplasma synoviae. Chapman Stone Agar .