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Handbook of Microbiological Media, Fourth Edition part 26 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Brain Heart Infusion Agar with 1 Agar HiVeg 245 Use For the cultivation of a wide variety of fastidious microorganisms including bacteria yeasts and molds. With the addition of 10 sheep blood it is used for the isolation and cultivation of many fungal species including systemic fungi from clinical and nonclinical specimens. The addition of gentamicin and chloramphenicol with 10 sheep blood produces a selective medium used for the isolation of pathogenic fungi from specimens heavily contaminated with bacteria and saprophytic fungi. It is recommended for the isolation of Histoplasma capsulatum and other pathogenic fungi including Coccidioides immitis. Brain Heart Infusion Agar BAM M24 Medium 2 Composition per liter Agar.15.0g Pancreatic digest of gelatin.14.5g Brain heart solids from infusion.6.0g Peptic digest of animal tissue.6.0g NaCl.5.0g Glucose.3.0g Na2HPO4.2.5g pH 7.4 0.2 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks while shaking to distribute precipitate. Autoclave for 15 min at 15 psi pressure-121 C. Mix thoroughly. Pour into sterile Petri dishes. Use For the cultivation of a wide variety of fastidious microorganisms including bacteria yeasts and molds. Brain Heart Infusion Agar 0.7 BHI Agar 0.7 BAM M23 Composition per liter Beef heart infusion.250.0g Calf brain infusion.200.0g Proteose peptone.10.0g Agar.7.0g NaCl.5.0g Na2HPO4-12H2O.2.5g Glucose.2.0g pH 5.3 0.2 at 25 C Source This medium without agar is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium Add components except agar to dis-tilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.3 with 1jV HCl. Mix thoroughly. Add agar. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 10 min at 15 psi pressure-121 C. Use For the detection of staphylococcal
