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Handbook of Microbiological Media, Fourth Edition part 21

Handbook of Microbiological Media, Fourth Edition part 21 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | BAM SMAgar Modified 195 Filter. To filtrate add an equal volume of 0.067M potassium phosphate buffer pH 7.5. Add 1.0g of dried liver concentrate. Mix thoroughly. Distribute into tubes or flasks in 10.0mL volumes. Autoclave for 20 min at 15 psi pressure-121 C. Prior to inoculation add 0.01g of rice starch to each tube. Use For the cultivation and maintenance of Entamoeba histolytica and other intestinal protozoa. BAM Agar ATCC Medium 1655 Composition per liter Agar.30.0g Glucose.5.0g KH2PO4.3.0g Yeast extract.1.0g MgSO4-7H2O.0.5g CaCl22H2O.0.25g NH4 2SO4.0.2g Trace elements .1.0mL pH 4.0 0.2 at 25 C Trace Elements Composition per liter CaCl22H2O.0.66g Na2MoO4-2H2O.0.3g ZnSO4-7H2O.0.18g CoCl26H2O.0.18g CuSO4-5H2O.0.16g MnSO4-4H2O.0.15g H3BO3.0.1g Preparation of Trace Elements Add components to 1.0L of dis-tilled deionized water. Mix thoroughly. Preparation of Medium Add components except agar to dis-tilled deionized water and bring volume to 800.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust medium to pH 4.0 with H2SO4. Add agar to 200.0mL of distilled deionized water. Autoclave agar separately to avoid acid hydrolysis. Autoclave for 15 min at 15 psi pres-sure-121 C. Mix the two solutions together. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and maintenance of Bacillus acidoterrestris. BAM Broth Composition per liter Glucose.5.0g KH2PO4.3.0g Yeast extract.1.0g MgSO4-7H2O.0.5g CaCl22H2O.0.25g NH4 2SO4.0.2g Trace elements .1.0mL pH 4.0 0.2 at 25 C Trace Elements Composition per liter CaCl22H2O.0.66g Na2MoO4-2H2O.0.3g ZnSO4-7H2O.0.18g CoCl26H2O.0.18g CuSO4-5H2O.0.16g MnSO4-4H2O.0.15g H3BO3 . 0.1g Preparation of Trace Elements Add components to 1.0L of dis-tilled deionized water. Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust medium to pH 4.0 with H2SO4. Distribute into tubes or flasks. Autoclave