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Handbook of Microbiological Media, Fourth Edition part 18 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Azospirillum Medium 165 Bromthymol Blue Solution Composition per 100.0mL Bromthymol Blue.0.5g Preparation of Bromthymol Blue Solution Add Bromthymol Blue to 100.0mL of 0.2N KOH. Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of Azospirillum amazon-ense. Azospirillum lipoferum Agar Medium Composition per liter Glucose.20.0g Agar.15.0g K2HPO4.0.8g MgSO4-7H2O.0.5g KH2PO4.0.2g FeCl3-6H2O.0.1g Yeast extract.0.1g CaCl22H2O.0.02g Na2MoO4-2H2O.0.02g pH 6.9 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of Azospirillum lipoferum. Azospirillum lipoferum Agar Medium Composition per liter Agar.15.0g Calcium malate.10.0g K2HPO4.0.8g MgSO4-7H2O.0.5g KH2PO4.0.2g FeCl3-6H2O.0.1g Yeast extract.0.1g CaCl22H2O.0.02g Na2MoO4-2H2O.0.02g pH 6.9 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of Azospirillum lipoferum. Azospirillum lipoferum Medium Composition per liter Calcium malate.10.0g K2HPO4.1.0g MgSO4-7H2O.0.5g CaCl22H2O.0.02g pH 6.5 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the isolation and cultivation of Azospirillum lipoferum.