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Handbook of Microbiological Media, Fourth Edition part 17 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | ASW Medium 155 Solution A Composition per 500.0mL NaNO3.6.0g KCl.1.52g KH2PO4.1.52g Preparation of Solution A Add components to distilled deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 6.5 with 2N NaOH. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Solution B Composition per 250.0mL Agar.15.0g MgSO4-7H2O.0.52g FeSO4-7H2O.1.0pg ZnSO4-7H2O.1.0pg Preparation of Solution B Add components to distilled deionized water and bring volume to 250.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Solution C Composition per 200.0mL Glucose.10.0g Preparation of Solution C Add glucose to distilled deionized water and bring volume to 200.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Preparation of Medium Aseptically combine sterile solution A sterile solution B and sterile solution C. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and maintenance of Aspergillus nidulans. Aspergillus Test Medium Composition per liter Agar.20.0g Malt extract.20.0g Peptone.1.0g Glucose solution.100.0mL Supplement solution.100.0mL pH 6.5 0.2 at 25 C Glucose Solution Composition per 100.0mL Glucose.20.0g Preparation of Glucose Solution Add glucose to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Warm to 50 C. Supplement Solution Composition per 100.0mL Uridine.2.44g Arginine.200.0mg Methionine.50.0mg Riboflavin.2.5mg Nicotinic acid.2.0mg -Aminobenzoic acid.1.0mg Pyrdoxine-HCl.0.05mg Biotin.0.02mg Preparation of Supplement Solution Add components to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Warm to 50 C. Preparation of Medium Add components except glucose solution and supplement solution to distilled deionized water and bring volume to 800.0mL. Mix thoroughly. Adjust pH to 6.5. Gently heat and bring to .
