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Handbook of Microbiological Media, Fourth Edition part 71 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Fluorocult E. coli O157 H7 Agar 695 Fluid Thioglycolate Medium with Rabbit Serum Composition per liter Pancreatic digest of casein.15.0g Glucose.5.5g Yeast extract.5.0g NaCl.2.5g Agar.0.75g L-Cystine.0.5g Sodium thioglycolate.0.5g Resazurin.1.0mg Rabbit serum.100.0mL pH 7.1 0.2 at 25 C Preparation of Medium Add components except rabbit serum to distilled deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 9.0mL volumes. Autoclave for 15 min at 15 psi pressure-121 C. If medium becomes oxidized before use resazurin turns red heat in a boiling water bath to expel absorbed O2. Cool to 25 C. Immediately prior to inoculation aseptically and anaerobically add 1.0mL of sterile rabbit serum to each tube. Mix thoroughly. Use For the cultivation of anaerobic microaerophilic and aerobic microorganisms. For use in the sterility testing of a variety of specimens. Fluid Thioglycolate Medium without Glucose or Eh Indicator Composition per liter Pancreatic digest of casein.20.0g NaCl.2.5g Agar.0.75g L-Cystine.0.5g Sodium thioglycolate.0.5g pH 7.1 0.2 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically distribute into sterile screw-capped tubes or flasks. Use For cultivation of anaerobic bacteria. For use as a basal medium in carbohydrate fermentation tests for differentiating anaerobic bacteria. For carbohydrate fermentation tests 1.0 mL of a 10 filter-sterilized carbohydrate solution is aseptically added to 9.0mL of fluid thioglycolate medium. Fluorescence Denitrification Medium See FN Medium Fluorescence Lactose Nitrate Medium See FLN Medium Fluorescent Pectolytic Agar FPA Medium Composition per liter Proteose peptone No. 3.20.0g Agar.15.0g .
