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Handbook of Microbiological Media, Fourth Edition part 8

Handbook of Microbiological Media, Fourth Edition part 8 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | AH5 Medium 65 Preparation of Medium Add components except cycloheximide solution and triphenyl tetrazolium chloride solution D-cycloserine solution and trimethoprim solution to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Distribute 100.0mL into flasks. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptically add per 100.0mL of medium 0.1 mL sterile triphenyl tetrazolium chloride solution 0.1mL sterile D-cycloserine solution 0.1mL sterile trimethoprim solution and 1.0mL cycloheximide solution. Mix thoroughly. Aseptically pour into sterile Petri dishes. Use For the selective cultivation of Agrobacterium tumefaciens biovar 3. Agrobacterium tumefaciens Selective Medium Composition per 1020mL Agar.15.0g L - Arabitol.3.04g K2HPO4.1.04g KH2PO4.0.54g Sodium taurocholate.0.29g MgSO4-7H2O.0.25g NH4NO3.0.16g Cycloheximide solution.10.0mL Selenite solution.10.0mL Crystal Violet 0.1 solution .2.0mL Selenite Solution Composition per 10.0mL NaOH.0.5g Na2SeO3-5H2O.0.1g Preparation of Selenite Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Cycloheximide Solution Composition per 10.0mL Cycloheximide.0.02g Preparation of Cycloheximide Solution Add cycloheximide to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Caution Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation. Preparation of Medium Add components except cycloheximide solution and selenite solution to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute 100.0mL into flasks. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptically add per 100.0mL of medium 1.0mL sterile selenite solution and 1.0mL cycloheximide solution. Mix thoroughly. Aseptically pour into sterile Petri dishes. Use For the selective cultivation of Agrobacterium