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Handbook of Microbiological Media, Fourth Edition part 5 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Acetobacterium 2 Medium 35 Wolfe s Mineral Solution Composition per liter MgSO4-7H2O.3.0g Nitrilotriacetic acid.1.5g NaCl.1.0g MnSO4-2H2O.0.5g CoCl2-6H2O.0.1g ZnSO4-7H2O.0.1g CaCl22H2O.0.1g FeSO4-7H2O.0.1g NiCl26H2O.0.025g KAl SO4 212H2O.0.02g CuSO4-5H2O.0.01g H3BO3.0.01g Na2MoO4-2H2O.0.01g Na2SeO3-5H2O.0.3mg Preparation of Wolfe s Mineral Solution Add nitrilotriacetic acid to 500.0mL of distilled deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled deionized water to 1.0L. Adjust pH to 6.8. NaHCO3 Solution Composition per 10.0mL NaHCO3.1.0g Preparation of NaHCO3 Solution Add NaHCO3 to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Na2S-9H2O Solution Composition per 10.0mL Na2S-9H2O.0.7g Preparation of Na2S-9H2O Solution Add Na2S-9H2O to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Before use neutralize to pH 7.0 with sterile HCl. Preparation of Medium Prepare and dispense medium under 100 N2. Add components except fructose solution NaHCO3 solution and Na2S-9H2O solution to distilled deionized water and bring volume to 880.0mL. Mix thoroughly. Adjust pH to 6.8-7.0. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically add 100.0mL of sterile fructose solution 10.0mL of sterile NaHCO3 solution and 10.0mL of sterile Na2S-9H2O solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or bottles. Use For the cultivation of Acetobacterium bakii and Acetobacterium paludosum. Acetobacterium 2 Medium Composition per 1050.0mL NaCl.20.0g NaHCO3.10.0g Yeast extract.2.0g Ethylene glycol.1.25g NH4Cl.1.0g K2HPO4.0.45g KH2PO4.0.33g MgSO4-7H2O.0.1g Resazurin.1.0mg Ethylene glycol solution.20.0mL Trace elements solution.20.0mL Vitamin solution.20.0mL Reducing agent solution.10.0mL Trace Elements .
