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Handbook of Microbiological Media, Fourth Edition part 4

Handbook of Microbiological Media, Fourth Edition part 4 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Acetivibrio Desulfovibrio Medium 25 ionized water and bring volume to 970.0mL. Mix thoroughly. Add solid bicarbonate and equilibrate pH to 6.8-7 by gassing. Gently heat and bring to boiling. Continue boiling for 3 min. Cool to room temperature while sparging with 80 N2 20 CO2. Anaerobically distribute 9.7mL volumes into anaerobic tubes. Autoclave for 20 min at 15 psi pressure-121 C. Aseptically and anaerobically add 0.1mL of sterile glucose 0.1mL of sterile L-cysteine-HCl-H2O solution and 0.1mL of sterile Na2S-9H2O solution to each tube. Mix thoroughly. Use For the cultivation of Acetitomaculum ruminus. Acetivibrio cellulolyticus Medium Composition per 1170.0mL Cellobiose or cellulose MN 300 Whatman CF II Kleenex tissue paper or HCl-treated cotton .3.0g NaHCO3.2.0g L-Cysteine-HCl.0.25g Na2S-9H2O.0.25g FeSO4-7H2O.0.02g Resazurin.0.001g Mineral solution 1.75.0mL Mineral solution 2.75.0mL Cellobiose solution.50.0mL Trace elements solution.10.0mL Vitamin solution.10.0mL Reducing agent solution.10.0mL pH 7.2 0.2 at 25 C Mineral Solution 1 Composition per liter K2HPO4.3.9g Preparation of Mineral Solution 1 Add K2HPO4 to distilled de-ionized water and bring volume to 1.0L. Mix thoroughly. Mineral Solution 2 Composition per liter NH4 2SO4.6.0g K2HPO4.2.4g MgSO4-7H2O.1.2g CaCl22H2O.0.72g NaCl.0.59g Preparation of Mineral Solution 2 Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Cellobiose Solution Composition per 50.0mL D-Cellobiose.5.0g Preparation of Cellobiose Solution Add cellobiose to distilled deionized water and bring volume to 50.0mL. Mix thoroughly. Sparge under 100 N2 gas for 3 min. Filter sterilize. Store under N2 gas. Trace Elements Solution Composition per liter MgSO4-7H2O.3.0g Nitrilotriacetic acid.1.5g CaCl2-2H2O.1.0g NaCl.1.0g MnSO4-2H2O.0.5g CoSO4-7H2O.0.18g ZnSO47H2O.0.18g FeSO4-7H2O.0.1g NiCl2-6H2O.0.025g KAI SO4 2-12H2O.0.02g CuSO4-5H2O.0.01g H3BO3.0.01g Na2MoO4 2H2O.0.01g Na2SeO3-5H2O.0.3mg Preparation of Trace .