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Tham khảo tài liệu 'bio-mems technologies and applications - wang and soper (eds) part 15', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 422 Bio-MEMS Technologies and Applications a b c FIGURE 15.20 Single-cell enzymatic assay within an aqueous droplet in soybean oil. a A mast cell was encapsulated in an aqueous droplet that contained the fluorogenic substrate FDG. b Prior to photolysis of the cell there was little fluorescent product within the droplet because the intracellular enzyme P-galactosidase was physically separated from FDG by the cell membrane. c d After laser induced cell lysis c P-galactosidase catalyzed the formation of the product fluorescein which caused the droplet to become highly fluorescent d . Reprinted with permission from He M. Edgar J.S. Jeffries G.D.M. Lorenz R.M. Shelby J.P. and Chiu D.T. 2005 . Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Analytical Chemistry 77 6 1539-1544. 2005 American Chemical Society. A method which combined the optical trapping and microfluidic-based droplet generation was developed by He et al. 2005 . This work described a method for selective and controlled encapsulation of a single cell or sub-cellular structure into a picoliter or femtoliter volume of an aqueous droplet surrounded by an immiscible phase. Once the selected moiety was encased within the droplet rapid laser photolysis within the droplet was demonstrated Figure 15.20 . The process confined the cell lysate within the pL volume of the droplet. With further development the droplet manipulations could be combined with droplet fusion for initiating chemical reactions such as derivatization reactions. The biggest advantage of such an approach is that the cell lysate and cell biocomponents are confined within a small volume minimizing the dilution and diffusion of the analytes of interest. 15.3 Single-Molecule Detection in Microfluidic Devices Initial studies on SMD single-molecule detection in microfluidic devices have indicated how powerful this marriage can become Dorre et al. 1997 2007 by Taylor Francis Group LLC .
