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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Genome sequence and global sequence variation map with 5.5 million SNPs in Chinese rhesus macaque. | Fang et al. Genome Biology 2011 12 R63 http genomebiology.eom 2011 12 7 R63 Genome Biology RESEARCH Open Access Genome sequence and global sequence variation map with 5.5 million SNPs in Chinese rhesus macaque 1t X r 71 2.3t 2.4t 2.3 2.3 I X 2 X z 1 Xiaodong Fang Yanfeng Zhang Rui Zhang Lixin Yang Ming Li Kaixiong Ye Xiaosen Guo Jun Wang1 and Bing Su2 Abstract Background Rhesus macaque Macaca mulatta is the most widely used nonhuman primate animal in biomedical research. A global map of genetic variations in rhesus macaque is valuable for both evolutionary and functional studies. Results Using next-generation sequencing technology we sequenced a Chinese rhesus macaque genome with 11.56-fold coverage. In total 96 of the reference Indian macaque genome was covered by at least one read and we identified 2.56 million homozygous and 2.94 million heterozygous SNPs. We also detected a total of 125 150 structural variations of which 123 610 were deletions with a median length of 184 bp ranging from 25 bp to 10 kb 63 of these deletions were located in intergenic regions and 35 in intronic regions. We further annotated 5 187 and 962 nonsynonymous SNPs to the macaque orthologs of human disease and drug-target genes respectively. Finally we set up a genome-wide genetic variation database with the use of Gbrowse. Conclusions Genome sequencing and construction of a global sequence variation map in Chinese rhesus macaque with the concomitant database provide applicable resources for evolutionary and biomedical research. Background Rhesus macaque Macaco mulatto and human shared a most recent common ancestor about 25 million years ago 1 and their genome sequences share 93.5 identity 2 . Due to the genetic and physiologic similarity between rhesus macaque and human rhesus macaques are the most widely used nonhuman primate animals for biomedical research for example in vaccine development and as animal models for human diseases 3-7 . In research rhesus macaque subspecies from India .
