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One of the important peptide hormones that control reproduction in crus-taceans is gonad-inhibiting hormone (GIH). GIH is known to modulate gonad maturation by inhibiting synthesis of vitellogenin (Vg), the precursor of yolk proteins. In this study, a cDNA encoding a GIH (Pem-GIH) from the eyestalk of Penaeus monodonwas cloned using RT-PCR and RACE techniques. | ễFEBS Journal Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference Supattra Treerattrakool1 Sakol Panyim1 2 Siu-Ming Chan3 Boonsirm Withyachumnarnkul4 5 and Apinunt Udomkit1 1 Institute of Molecular Biology and Genetics MahidolUniversity Nakhon Pathom Thailand 2 Department of Biochemistry MahidolUniversity Bangkok Thailand 3 Schoolof BiologicalSciences The University of Hong Kong China 4 Department of Anatomy MahidolUniversity Bangkok Thailand 5 Centex Shrimp MahidolUniversity Bangkok Thailand Keywords black tiger shrimp ovarian maturation reproduction RNA interference vitellogenesis Correspondence A. Udomkit Institute of Molecular Biology and Genetics MahidolUniversity Salaya Campus Nakhon Pathom 73170 Thailand Fax 66 2 441 9906 Tel 66 2 800 3624 ext. 1236 E-mail staud@mahidol.ac.th Received 16 November 2007 revised 18 December 2007 accepted 25 December 2007 doi 10.1111 j.1742-4658.2008.06266.x One of the important peptide hormones that control reproduction in crustaceans is gonad-inhibiting hormone GIH . GIH is known to modulate gonad maturation by inhibiting synthesis of vitellogenin Vg the precursor of yolk proteins. In this study a cDNA encoding a GIH Pem-GIH from the eyestalk of Penaeus monodon was cloned using RT-PCR and RACE techniques. Pem-GIH cDNA is 861 bp in size with a single ORF of 288 bp. The deduced Pem-GIH consists of a 17-residue signal peptide and a mature peptide region of 79 amino acids with features typical of type II peptide hormones from the CHH family. Pem-GIH transcript was detected in eyestalk brain thoracic and abdominal nerve cords of adult P. monodon. The gonad-inhibiting activity of Pem-GIH was investigated using the RNA interference technique. Double-stranded RNA corresponding to the mature Pem-GIH sequence can trigger a decrease in Pem-GIH transcript levels both in eyestalk ganglia and abdominal nerve cord explant culture and .
