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Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: " Effects of ectomycorrhizal inoculation and the type of substrate on mycorrhization, growth and nutrition of containerised Pinus pinea L. seedlings produced in a commercial nursery" | Ann. For. Sci. 62 2005 817-822 INRA EDP Sciences 2005 DOI 10.1051 forest 2005087 817 Original article Effects of ectomycorrhizal inoculation and the type of substrate on mycorrhization growth and nutrition of containerised Pinus pinea L. seedlings produced in a commercial nursery Ana RINCÓNa Javier PARLADÉb Joan PERAb a Departamento de Fisiología y Bioquímica Vegetal Centro de Ciencias Medioambientales CCMA-CSIC C Serrano 115 dupl. 28006 Madrid Spain b Departament de Protecció Vegetal Institut de Recerca i Tecnologia Agroalimentaries IRTA Centre de Cabrils Ctra. de Cabrils s n 08348 Cabrils Barcelona Spain Received 3 January 2005 accepted 22 August 2005 Abstract - Spore inocula of Melanogaster ambiguus Vittad. Tul. C. Tul. Pisolithus tinctorius Pers. Coker Couch Rhizopogon luteolus Fr. Rhizopogon roseolus Corda Th. M. Fr. and Scleroderma verrucosum Bull. Pers. and two substrates a mixture of peat-vermiculite and a mixture of peat-composted pine bark were evaluated for producing mycorrhizal Pinus pinea L. seedlings in a commercial nursery in NE Spain. Inocula of all fungi were effective to obtain containerised mycorrhizal P. pinea seedlings although seedling mycorrhization rates were reduced in the peat pine-bark mixture. Neither inoculation nor the type of substrate modified the first-year growth of seedlings. Rhizopogon spp. increased the levels of N and P and in general inoculation reduced the concentration of Mn in needles. Symptoms of chlorosis were detected in all seedlings growing in the peat pine-bark mixture probably due to nutritional deficiencies caused by the substrate high pH. Among the fungi tested R. roseolus is proposed as the best candidate for its application in nursery inoculation programmes destined to produced containerised mycorrhizal P. pinea. Pinus pinea substrate mycorrhization ectomycorrhizal fungi seedling nursery production Résumé - Effets d une inoculation ectomycorhizienne et du type de substrat sur la mycorhization la croissance et la