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Tham khảo tài liệu 'aquaculture part 7', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Novel Approach for Controlling Lipid Oxidation and Melanosis in Aquacultured Fish and Crustaceans Application of Edible Mushroom Flammulina velutipes Extract In Vivo 171 Fig. 8. Development of melanosis a and changes in the mean gray values of the carapace area b of kuruma shrimps Marsupenaeus japonicus during ice storage. Results are presented interms of mean standard deviation n 3 . The values with different superscript letters represent significant difference between groups for different storage periods p 0.05 Encarnacion et al. 2010 . Fig. 9. a Enzymatic oxidation of catechol and L-DOPA b in the hemolymph of kuruma shrimps Marsupenaeus japonicus after 7 days of feeding. Results are presented in terms of mean standard deviation n 4 . The values with different superscript letters represent significant difference p 0.05 at the end of the reaction period for each assay. b Gene expression analysis of prophenoloxidase proPO transcripts in the hemocytes of hemolymphs of kuruma shrimp M. japonicus after 7 days of feeding representative gel and quantitative analyses of band intensities obtained using the ImageJ software. Elongation factor1-a gene EF1-a was used as the standard for computing relative gene expression level for each sample band. Results are presented in terms of mean standard deviation n 3 . The values with different superscript letters represent significant difference p 0.05 Encarnacion et al. 2010 . 172 Aquaculture hemocytes of the control samples showing that the ME inhibited PPO activation in the hemolymphs of the supplement-fed shrimps Fig. 9 . Because PPO activity mainly depends on the activation of the proPO system decreasing the expression of proPO genes in hemocytes consequently reduces the activity of PPO. The results of a study performed by Amparyup et al. 2009 indicated the significant role of the proPO activating system as one of the major immune responses in shrimps and also reported that inhibiting proPO activation in shrimps could cause .