Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Whole-genome resequencing of Escherichia coli K-12 MG1655 undergoing short-term laboratory evolution in lactate minimal media reveals flexible selection of adaptive mutations. | Open Access Researc h Whole-genome resequencing of Escherichia coli K-12 MG1655 undergoing short-term laboratory evolution in lactate minimal media reveals flexible selection of adaptive mutations Tom M Conrad Andrew R Joyce M Kenyon Applebee Christian L Barrett Bin Xie Yuan Gao and Bernhard 0 Palsson Addresses Department of Chemistry and Biochemistry University of California San Diego 9500 Gilman Drive La Jolla California 920930332 USA. Department of Bioengineering University of California San Diego 9500 Gilman Drive La Jolla California 92093-0412 USA. Department of Computer Science Virginia Commonwealth University 401 West Main Street Richmond Virginia 23284-3019 USA. Center for the Study of Biological Complexity Virginia Commonwealth University 1000 W. Cary St. Richmond Virginia 23284-3068 USA. Correspondence Bernhard 0 Palsson. Email bpalsson@bioeng.ucsd.edu Published 22 October 2009 Genome Biology 2009 10 R118 doi 10.1186 gb-2009-10-10-r118 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2009 10 10 R118 Received 20 February 2009 Revised 18 September 2009 Accepted 22 October 2009 2009 Conrad et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited Abstract Background Short-term laboratory evolution of bacteria followed by genomic sequencing provides insight into the mechanism of adaptive evolution such as the number of mutations needed for adaptation genotype-phenotype relationships and the reproducibility of adaptive outcomes. Results In the present study we describe the genome sequencing of 11 endpoints of Escherichia coli that underwent 60-day laboratory adaptive evolution under growth rate selection pressure in lactate minimal media. Two to eight mutations .
