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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Importance of disulphide bonds for vaccinia virus L1R protein function | Virology Journal BioMed Central Short report Open Access Importance of disulphide bonds for vaccinia virus LIR protein function Robert E Blouch1 Chelsea M Byrd2 and Dennis E Hruby 1 2 Address department of Microbiology Oregon State University 220 Nash Hall Corvallis Oregon 97331 USA and 2Siga Technologies 4575 SW Research Way Suite 230 Corvallis Oregon 97333 USA Email Robert E Blouch - prb101@aol.com Chelsea M Byrd - cbyrd@sgph.com Dennis E Hruby - dhruby@sgph.com Corresponding author Published 09 December 2005 Received 09 August 2005 Accepted 09 December 2005 Virology Journal 2005 2 91 doi 10.1186 1743-422X-2-91 This article is available from http www.virologyj.com content 2 1 91 2005 Blouch et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract L1R a myristylated late gene product of vaccinia virus is essential for formation of infectious intracellular mature virions IMV . In its absence only viral particles arrested at an immature stage are detected and no infectious progeny virus is produced. Previous studies have shown that the L1R protein is exclusively associated with the IMV membrane and that myristylation is required for correct targeting. The L1R protein contains six cysteine amino acid residues that have all been shown to participate in intramolecular disulphide bonds. However it was not clear what role if any the disulfide bonds play in the membrane topology of the L1R protein. To address this question a comprehensive library of L1R mutants in which the cysteine residues have been mutated to serine either individually or in combination were tested for their ability to rescue a L1R conditional lethal mutant virus under non-permissive conditions. Much to our surprise we determined that C57 was not .
