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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Permissive human cytomegalovirus infection of a first trimester extravillous cytotrophoblast cell line | Virology Journal BioMed Central Short report Open Access Permissive human cytomegalovirus infection of a first trimester extravillous cytotrophoblast cell line Heather L LaMarca1 2 Bruno Sainz Jr2 and Cindy A Morris 1 2 Address 1Interdisciplinary Program in Molecular and Cellular Biology Tulane University Health Sciences Center New Orleans LA USA and 2Department of Microbiology and Immunology Tulane University Health Sciences Center New Orleans LA USA Email Heather L LaMarca - hlamarc@tulane.edu Bruno Sainz - bsainz@tulane.edu Cindy AMorris - cmorris2@tulane.edu Corresponding author Published 17 November 2004 Received 02 September 2004 Accepted 17 November 2004 Virology Journal 2004 1 8 doi 10.1186 I743-422X-I-8 This article is available from http www.virologyj.com content 1 1 8 2004 LaMarca et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Human cytomegalovirus HCMV is the leading cause of congenital viral infection in the United States and Europe. Despite the significant morbidity associated with prenatal HCMV infection little is known about how the virus infects the fetus during pregnancy. To date primary human cytotrophoblasts CTBs have been utilized to study placental HCMV infection and replication however the minimal mitotic potential of these cells restricts experimentation to a few days which may be problematic for mechanistic studies of the slow-replicating virus. The aim of this study was to determine whether the human first trimester CTB cell line SGHPL-4 was permissive for HCMV infection and therefore could overcome such limitations. HCMV immediate early IE protein expression was detected as early as 3 hours post-infection in SGHPL-4 cells and progressively increased as a function of time. HCMV .
