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Insect development is driven by the action of ecdysteroids on morphogenetic processes. The classic ecdysteroid receptor is a protein heterodimer com-posed of two nuclear receptors, the ecdysone receptor (EcR) and Ultraspira-cle (USP), the insect ortholog of retinoid X receptor. The functional properties of EcR and USP vary among insect species, and provide a basis for identifying novel and species-specific insecticidal candidates that disrupt this receptor’s normal activity. | ỊFEBS Journal Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system - a basis for screening novel insecticidal candidates Joshua M. Beatty1 Guy Smagghe2 Takehiko Ogura3 Yoshiaki Nakagawa3 Margarethe Spindler-Barth4 and Vincent C. Henrich1 1 Center for Biotechnology Genomics and Health Research University of North Carolina at Greensboro NC USA 2 Laboratory of Agrozoology Department of Crop Protection Faculty of Bioscience Engineering Ghent University Belgium 3 Division of Applied Life Sciences Graduate Schoolof Agriculture Kyoto University Japan 4 Institute of GeneralZoology and Endocrinology University of Ulm Germany Keywords cell culture Drosophila insecticide juvenile hormone nonsteroidalagonist Correspondence V. C. Henrich Center for Biotechnology Genomics and Health Research 1111 Spring Garden St University of North Carolina at Greensboro Greensboro NC 27402 USA Fax 1 336 334 4794 Tel 1 336 334 4775 E-mail vincent_henrich@uncg.edu Received 25 February 2009 revised 24 March 2009 Accepted 27 March 2009 doi 10.1111 j.1742-4658.2009.07026.x Insect development is driven by the action of ecdysteroids on morphogenetic processes. The classic ecdysteroid receptor is a protein heterodimer composed of two nuclear receptors the ecdysone receptor EcR and Ultraspiracle USP the insect ortholog of retinoid X receptor. The functional properties of EcR and USP vary among insect species and provide a basis for identifying novel and species-specific insecticidal candidates that disrupt this receptor s normal activity. A heterologous mammalian cell culture assay was used to assess the transcriptional activity of the heterodimeric ecdyster-oid receptor from species representing two major insect orders the fruit fly Drosophila melanogaster Diptera and the Colorado potato beetle Leptino-tarsa decemlineata Coleoptera . Several nonsteroidal agonists evoked a strong response with the L. decemlineata heterodimer that was consistent with .
