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Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Cloning of canine GM - CSF and SCF genes | J. Vet. Sei. 2001 2 3 159-166 IQDEDEBDB Veterinary Science Cloning of canine GM-CSF and SCF genes Il-seob Shin Myung-jin Nam1 Seong-Jun Park2 Hwa-young Youn and Hong-ryul Han Department of Veterinary Internal Medicine College of Veterinary Medicine and School of Agricultural Biotechnology Seoul National University Seoul 151-742 Korea. 1 Cancer Research Division National Institute of Health Seoul 122-701 Korea 2Department of Veterinary Internal Medicine Tokyo University of Agriculture and Technology 3-5-8 Saiwai-cho Fuchu Tokyo 183-8509 Japan. ABSTRACT Cytokines have pleiotropic regulatory effects on hematopoietic cells and many other cell types that participate in host defence and repair processes. Granulocyte-macrophage colony-stimulating factor GM-CSF mediates the growth and differentiation of granulocytes and macrophages and regulates the biological functions expressed by mature cells of these lineages. Stem cell factor SCF is a multifunctional cytokine involved in hematopoiesis melanogenesis and gametogenesis. In order to determine the complementary DNA cDNA of canine GM-CSF and canine SCF cDNA clones were generated from lipopolysaccharide IPS stimulated peripheral blood mononuclear cells PBMCs and bone marrow cells by reverse transcription PCR amplification. The canine GM-CSF cDNA obtained in this study contains an open reading frame encoding 144 amino acid residues and has 53-75 homology with those of human cat sheep pig cow and mouse. Canine SCF cDNA consist of an open reading frame encoding 274 amino acid residues and shares 81-92 homology with those of human cat pig cow and mouse. Key word cytokine cDNA cloning PCR GM-CSF SCF dog INTRODUCTION Cytokines are regulatory peptides that are produced by This research was performed as a part of Studies of cocktail therapy with multiple cytokines for neoplasia or infectious diseases of the dog with the grants supported by Korean Research Foundation. C MTesponding author Phone 82-2-880-8682 Fax 82-2-880-8682 E-mail
