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We investigated the functional activity of recombinant Bax inhibitor-1 reconstituted into liposomes. When proteoliposomes were suspended in acidic solutions, encapsulated Ca 2+ was released from the membranes, as previously suggested [Kim HR, Lee GH, Ha KC, Ahn T, Moon JY, Lee BJ, Cho SG, Kim S, Seo YR, Shin YJet al. (2008) J Biol Chem 283, 15946–15955]. | ỊFEBS Journal Ca2 H antiporter-like activity of human recombinant Bax inhibitor-1 reconstituted into liposomes Taeho Ahn1 Chul-Ho Yun2 Ho Zoon Chae2 Hyung-Ryong Kim3 and Han-Jung Chae4 1 Department of Biochemistry College of Veterinary Medicine Chonnam National university Gwangju Korea 2 Schoolof BiologicalSciences and Technology Chonnam NationalUniversity Gwangju Korea 3 Department of DentalPharmacology Schoolof Dentistry Wonkwang University Iksan Korea 4 Department of Pharmacology and Institute of Cardiovascular Research Medical School Chonbuk NationalUniversity Jeonju Korea Keywords antiporter Bax inhibitor-1 Ca2 -release proteoliposome reconstitution Correspondence T. Ahn Department of Biochemistry College of Veterinary Medicine Chonnam NationalUniversity Gwangju 500-757 Korea Fax 82 62 530 2809 Tel 82 62 530 2823 E-mail thahn@chonnam.ac.kr H.-J. Chae Department of Pharmacology and Institute of Cardiovascular Research Medical School Chonbuk University Jeonju Chonbuk 561-181 Korea Fax 82 63 275 2855 Tel 82 63 270 3092 E-mail hjchae@chonbuk.ac.kr Received 11 November 2008 revised 30 December 2008 accepted 10 February 2009 doi 10.1111 j.1742-4658.2009.06956.x We investigated the functional activity of recombinant Bax inhibitor-1 reconstituted into liposomes. When proteoliposomes were suspended in acidic solutions encapsulated Ca2 was released from the membranes as previously suggested Kim HR Lee GH Ha KC Ahn T Moon JY Lee BJ Cho SG Kim S Seo YR Shin YJ et al. 2008 J Biol Chem 283 15946-15955 . Concomitantly proton ions were internalized when assayed using the time-dependent change in the fluorescence of the pH-sensitive dye oxonol V entrapped in the proteoliposomes. The influx of proton ions was confirmed by observing tritium accumulation in the membranes. However the external acidity of the membranes per se did not induce proton ion influx without internalized Ca2 . These results suggest that reconstituted Bax inhibitor-1 has a Ca2 H antiporter-like activity. Bax
