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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Usefulness of Herpes Consensus PCR methodology to routine diagnostic testing for herpesviruses infections in clinical specimens | Virology Journal BioMed Central Short report Open Access Usefulness of Herpes Consensus PCR methodology to routine diagnostic testing for herpesviruses infections in clinical specimens Georgia Vrioni 1 Christos Kalogeropoulos2 Constantina Gartzonika1 Efthalia Priavali1 and Stamatina Levidiotou1 Address Department of Microbiology Medical School University of Ioannina Greece and 2Department of Ophthalmology University Eye Clinic of loannina Greece Email Georgia Vrioni - gvrioni@med.uoa.gr Christos Kalogeropoulos - chkalog@cc.uoi.gr Constantina Gartzonika - kgartzon@cc.uoi.gr Efthalia Priavali - epriaval@cc.uoi.gr Stamatina Levidiotou - sleveidi@uoi.gr Corresponding author Published 12 June 2007 Received 5 December 2006 Accepted 12 June 2007 Virology Journal 2007 4 59 doi l0.ll86 l 743-422X-4-59 This article is available from http www.virologyj.cOm content 4 1 59 2007 Vrioni et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The purposes of the study were to assess the usefulness of simultaneously amplifying herpes simplex virus 1 and 2 varicella-zoster virus cytomegalovirus Epstein-Barr virus and human herpesvirus 6 DNA in various clinical specimens and to analyze clinical events in patients presenting positive results. A total of 763 clinical samples obtained from 758 patients including ll5 cerebrospinal fluids l02 aqueous fluids 445 swabs from genital l52 oro-facial l38 and other 155 skin lesions 96 eye swabs and 5 bronchoalveolar lavages were tested by using the Consensus polymerase chain reaction methodology. The clinical files of the patients were consulted retrospectively. l7l of the 758 patients 22.5 were positive for at least one of the six target viruses herpes simplex virus l n 95 varicella-zoster virus