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Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery | Genetic Vaccines and Therapy BioMed Central Research Open Access Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery Weidong Zhang1 Rajeswari Singam1 Gary Hellermann1 Xiaoyuan Kong1 Homero San Juan1 Richard F Lockey1 Shuen-Ju Wu2 Kevin Porter2 and Shyam S Mohapatra 1 Address Division of Allergy and Immunology-JMC Airway Disease Research Center Department of Internal Medicine University of South Florida VA Hospital Tampa FL USA and 2Viral Diseases Department Naval Medical Research Center Silver Spring Maryland USA Email Weidong Zhang - zwdjohn@yahoo.com Rajeswari Singam - rsingam@hsc.usf.edu Gary Hellermann - ghellermann@yahoo.com Xiaoyuan Kong - xkong@hsc.usf.edu Homero San Juan - hsanjuan@hsc.usf.edu Richard F Lockey - rflockey@hsc.usf.edu Shuen- Ju Wu - wus@nmrc.navy.mil Kevin Porter - porterkr@nmrc.navy.mil Shyam S Mohapatra - smohapat@hsc.usf.edu Corresponding author Published 09 August 2004 Received 26 February 2004 Genetic Vaccines and Therapy 2004 2 8 doi 10.1 186 1479-0556-2-8 Accepted 09 August 2004 This article is available from http www.gvt-journal.cOm content 2 1 8 2004 Zhang et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The need for safe and effective treatment of dengue virus DEN a class A agent that causes dengue hemorrhagic fever dengue shock syndrome has been a critical global priority. An effective vaccine for DEN is not yet available. In this study the possibility of attenuating DEN infection using adeno-associated virus AAV -encoded short interfering RNAs siRNA was examined in Vero cells and human dendritic cells DCs . Methods A cassette encoding siRNA targeted to a 3 untranslated sequence common to all DEN serotypes was designed and tested
