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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution. | Research Open Access Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution Julia Tischler Ben Lehner Nansheng Chen and Andrew G Fraser Addresses The Wellcome Trust Sanger Institute Hinxton Cambridge CB10 1SA UK. CRG-EMBL Systems Biology Program Centre for Genomic Regulation Barcelona Spain. Molecular Biology and Biochemistry Simon Fraser University University Drive Burnaby British Columbia V5A 1S6 Canada. Correspondence AndrewG Fraser. Email agf@sanger.ac.uk Published 2 August 2006 Genome Biology 2006 7 R69 doi 10.1 186 gb-2006-7-8-r69 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2006 7 8 R69 Received 14 February 2006 Revised 7 June 2006 Accepted 2 August 2006 2006 Tischler et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Systematic analyses of loss-of-function phenotypes have been carried out for most genes in Saccharomyces cerevisiae Caenorhabditis elegans and Drosophila melanogaster. Although such studies vastly expand our knowledge of single gene function they do not address redundancy in genetic networks. Developing tools for the systematic mapping of genetic interactions is thus a key step in exploring the relationship between genotype and phenotype. Results We established conditions for RNA interference RNAi in C. elegans to target multiple genes simultaneously in a high-throughput setting. Using this approach we can detect the great majority of previously known synthetic genetic interactions. We used this assay to examine the redundancy of duplicated genes in the genome of C. elegans that correspond to single .