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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Analysis of cell-based RNAi screens. | Software Analysis of cell-based RNAi screens Michael Boutros Lígia P Bras and Wolfgang Huber Open Access Addresses Signaling and Functional Genomics German Cancer Research Center Im Neuenheimer Feld 580 69120 Heidelberg Germany. EMBL - European Bioinformatics Institute Cambridge CB10 1SD UK. Centre for Chemical and Biological Engineering 1ST Technical University of Lisbon Av. Rovisco Pais P-1049-001 Lisbon Portugal. Correspondence Michael Boutros. Email m.boutros@dkfz.de. Wolfgang Huber. Email huber@ebi.ac.uk Published 25 July 2006 Genome Biology 2006 7 R66 doi 10.1 186 gb-2006-7-7-r66 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2006 7 7 R66 Received 27 March 2006 Revised 7 June 2006 Accepted 25 July 2006 2006 Boutros et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract RNA interference RNAi screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor R package cellHTS http www.dkfz.de signaling cellHTS . The method is useful for the analysis and documentation of individual RNAi screens. Moreover it is a prerequisite for the integration of multiple experiments. Rationale RNA interference RNAi is a conserved biological mechanism to silence gene expression on the level of individual transcripts. RNAi was discovered in Caenorhabditis elegans when Fire and Mello 1 observed that injecting long doublestranded ds RNAs into worms led to efficient silencing of homologous endogenous RNAs. .
