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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: TANGOing along the protein secretion pathway. | Minireview TANGOing along the protein secretion pathway Catherine Rabouille and Vangelis Kondylis Address University Medical Center Utrecht The Cell Microscopy Center Department of Cell Biology and Institute of Biomembranes Heidelberglaan 100 3584CX Utrecht The Netherlands. Correspondence Catherine Rabouille. Email C.Rabouille@lab.azu.nl Published 28 April 2006 Genome Biology 2006 7 213 doi 10.1186 gb-2006-7-4-213 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2006 7 4 213 2006 BioMed Central Ltd Abstract Although the organization and functions of the constitutive secretory pathway have been intensively studied for decades a recent genome-wide RNAi screen in Drosophila cells has identified about 100 genes encoding novel so-called TANGO proteins for transport and Golgi organization that may be direct regulators of various aspects of protein exocytosis or secretion. Soon after the phenomenon of RNA interference RNAi was described in animal cells 1 it became evident that RNAi not only represented an ancient mechanism to control viral infections and physiological developmental processes but it would also provide researchers with a powerful tool for functional genetic analysis 2 . Because of its efficiency and specificity RNAi has gained ground as a method of disrupting protein function over the traditional method of transiently expressing dominant-negative mutant proteins which can yield misleading results 3 . In fact downregulation or depletion of a protein with unknown function by RNAi has nowadays become an almost obligatory part of its characterization. The availability of entire genome sequences of an increasing number of organisms has given RNAi an additional boost. The construction of double-stranded ds RNA libraries representing nearly all the genes in a genome provides the opportunity to identify proteins that regulate specific cellular and developmental processes. Several genome-wide RNAi screens .
