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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Release of extraction-resistant mRNA in stationary phase Saccharomyces cerevisiae produces a massive increase in transcript abundance in response to stress. | Research Open Access Release of extraction-resistant mRNA in stationary phase Saccharomyces cerevisiae produces a massive increase in transcript abundance in response to stress Anthony D AragonH Gabriel A Quinones Edward V Thomas Sushmita Roy and Margaret Werner-Washburne Addresses Department of Biology University of New Mexico Albuquerque NM 87131 USA. Cancer Biology Program Stanford University Stanford CA 94305 USA. Sandia National Laboratories Albuquerque NM 87185 USA. Department of Computer Science University of New Mexico Albuquerque NM 87131 USA. H These authors contributed equally to this work. Correspondence MargaretWerner-Washburne. Email maggieww@unm.edu Published 8 February 2006 Genome Biology 2006 7 R9 doi 10.1186 gb-2006-7-2-r9 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2006 7 2 R9 Received 23 September 2005 Revised 16 November 2005 Accepted l0 January 2006 2006 Aragon et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background As carbon sources are exhausted Saccharomyces cerevisiae cells exhibit reduced metabolic activity and cultures enter the stationary phase. We asked whether cells in stationary phase cultures respond to additional stress at the level of transcript abundance. Results Microarrays were used to quantify changes in transcript abundance in cells from stationary phase cultures in response to stress. More than 800 mRNAs increased in abundance by one minute after oxidative stress. A significant number of these mRNAs encode proteins involved in stress responses. We tested whether mRNA increases were due to new transcription rapid poly-adenylation of message which would not be detected by microarrays or potential .