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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Analysis of gene expression in operons of Streptomyces coelicolor. | Research Open Access Analysis of gene expression in operons of Streptomyces coelicolor Emma Laing Vassilis Mersinias Colin P Smith and Simon J Hubbard Addresses Faculty of Life Sciences The University of Manchester Manchester M13 9PT UK. Current Address School of Biomedical and Molecular Sciences University of Surrey Guildford GU2 7XH UK. Functional Genomics Laboratory School of Biomedical and Molecular Sciences University of Surrey Guildford GU2 7XH UK. Correspondence Simon J Hubbard. Email simon.hubbard@manchester.ac.uk Published 2 June 2006 Genome Biology 2006 7 R46 doi l0.l 186 gb-2006-7-6-r46 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2006 7 6 R46 Received 22 December 2005 Revised 3 March 2006 Accepted 9 May 2006 2006 Laing et al. licensee BioMed Central Ltd. This is an open acess article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Recent studies have shown that microarray-derived gene-expression data are useful for operon prediction. However it is apparent that genes within an operon do not conform to the simple notion that they have equal levels of expression. Results To investigate the relative transcript levels of intra-operonic genes we have used a Z-score approach to normalize the expression levels of all genes within an operon to expression of the first gene of that operon. Here we demonstrate that there is a general downward trend in expression from the first to the last gene in Streptomyces coelicolor operons in contrast to what we observe in Escherichia coli. Combining transcription-factor binding-site prediction with the identification of operonic genes that exhibited higher transcript levels than the first gene of the same operon enabled the discovery of putative internal .
