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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae. | Research Open Access Characterization of the yeast ionome a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae David J Eide Suzanne Clark T Murlidharan Nair Mathias Gehl Michael Gribskov Mary Lou Guerinot and Jeffrey F Harper Addresses Department of Nutritional Sciences University of Wisconsin-Madison Madison WI 53706 USA. San Diego Supercomputer Center University of California-San Diego La Jolla CA 92903 USA. Biochemistry Department University of Nevada Reno Nevada 89557 USA. Department of Biological Sciences Dartmouth College Hanover NH 03755 USA. Correspondence DavidJ Eide. E-mail eide@nutrisci.wisc.edu Published 30 August 2005 Genome Biology 2005 6 R77 doi 10.1 186 gb-2005-6-9-r77 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2005 6 9 R77 Received 29 March 2005 Revised 21 June 2005 Accepted 18 July 2005 2005 Eide et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Nutrient minerals are essential yet potentially toxic and homeostatic mechanisms are required to regulate their intracellular levels. We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae. Using inductively coupled plasma-atomic emission spectroscopy ICP-AES we assayed 4 385 mutant strains for the accumulation of 13 elements calcium cobalt copper iron potassium magnesium manganese nickel phosphorus selenium sodium sulfur and zinc . We refer to the resulting accumulation profile as the yeast ionome . Results We identified 212 strains that showed altered ionome profiles when grown on a rich growth medium. Surprisingly few of these mutants four strains were affected for